Mutational Analysis of Growth Differentiation Factor-9 Gene in Korean Women with Premature Ovarian Failure.
- Author:
Hee Ju GU
1
;
Young Moon KIM
;
Dong Wook JANG
;
Ji Young LEE
;
In Sook SOHN
;
Soo Nyung KIM
;
Ki Hyun PARK
;
Andrew R ZINN
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Konkuk University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Premature ovarian failure;
GDF-9 gene;
dHPLC
- MeSH:
Adult;
Amenorrhea;
Bone Morphogenetic Protein 15;
Chromatography, High Pressure Liquid;
Female;
Gonadotropins;
Growth Differentiation Factor 9;
Humans;
Inhibins;
Mass Screening;
Primary Ovarian Insufficiency*;
Receptors, FSH;
Receptors, LH
- From:Korean Journal of Obstetrics and Gynecology
2003;46(5):938-945
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The clinical models for studying ovary-determining genes may be premature ovarian failure (POF). POF is a condition causing amenorrhea, hypoestrogenism, and elevated gonadotropins in women under 40 years old. FSH receptor, LH receptor, inhibin, GDF-9 (growth differentiation factor-9), BMP-15 (bone morphogenetic protein-15), DIAPH2 (diaphanous gene) and XPNPEP2 (X-prolyl aminopeptidase) genes were proposed as a possible candidate gene, but until recently, only mutations in FSH receptor, LH receptor and inhibin genes have been identified in POF patients. Therefore mutation screening of another POF gene necessary to reveal the principal causative genes of POF. OBJECTIVE: The present study was performed to analyze the mutation of GDF-9 gene in Korean patient with POF and to investigate whether mutation of these gene is a likely main cause of POF. METHODS: Eighty-six women with POF were studied and thirty-six normal women were enrolled as control. Mutation screening of these genes were performed by denaturing HPLC and were confirmed by automatic sequencing. RESULTS: Three different mutations of GDF-9 gene were identified in Korean women with POF; Arg3Cys mutation in one patient, Leu40Val mutation in one patient, Asp57Tyr mutation in 10 patients and 5 normal controls. Arg3Cys mutation and Leu40Val mutation were likely cause of disease. Frequencies of Arg3Cys mutation and Leu40Val mutation were 1.2%, respectively. Asp57Tyr mutation was common polymorphism in Korean women. All mutations was a novel mutation found in the present study. CONCLUSION: POF was resulted by mutations of GDF-9 gene, but mutations of GDF-9 gene are not likely main causes of POF because of low frequency of mutations.
