Uptake and transport of biochanin A in Caco-2 cell monolayers：a comparison study between biochanin A and PEGylated biochanin A loaded nanostructured lipid carriers
- VernacularTitle:鹰嘴豆芽素 A及其聚乙二醇化纳米脂质载体在Caco-2细胞模型的摄取和转运机制研究
- Author:
Qing LUO
1
;
Lei WANG
;
Tong-Yuan LIN
;
Ting-Ting ZHU
;
Wei-Dong CHEN
Author Information
1. 安徽中医药大学
- Keywords:
biochanin A;
Caco-2 cell;
uptake;
transport
- From:
The Chinese Journal of Clinical Pharmacology
2014;(8):715-718
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the absorption mechanism of Bio-chanin A ( BCA) and PEGylated BCA loaded nanostructured lipid carri-ers ( PEG -BCA -NLC ) across Caco -2 cell model.Methods Caco-2 cell monolayer model was used to explore the uptake , transport and impact factors of BCA and PEG -BCA-NLC.Drug concentration was measured by HPLC and the apparent permeability coefficients ( Papp ) were calculated.Results The main mechanism of BCA and PEG-BCA-NLC across Caco -2 monolayer model was passive diffu-sion.But under the same time , lower uptake of drug in Caco -2 cells was observed at PEG -BCA -NLC group comparing to that at BCA group.The absorption and the apparent permeability coefficient of BCA in Caco-2 cells monolayer were increased when combined with verapam-il.The Papp (AP→BL) increased and Papp (BL→PL) reduced, but the uptake content was much lower than BCA solution.Conclusion Ab-sorption process of PEG -BCA-NLC in Caco-2 cells monolayer met the characteristics of passive diffusion.However , the PEG-BCA-NLC may not significantly improve the absorption of BCA in intestinal epithelial.
