Effects of interleukin-1βon the sensitivity of vascular smooth muscle cells to com-pactin treatments
- VernacularTitle:白细胞介素1β影响人血管平滑肌细胞对美伐他汀的敏感性
- Author:
Yong YAN
1
;
Guo-Qing ZUO
;
Ya-Xi CHEN
;
Xiong-Zhong RUAN
;
Lei ZHAO
Author Information
1. 重庆医科大学 脂糖代谢性疾病重庆市重点实验室
- Keywords:
interleukin-1β;
compactin;
vascular smooth muscle cell
- From:
The Chinese Journal of Clinical Pharmacology
2014;(8):701-703,711
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of interleukin -1β( IL-1β) on the sensitivity of human vascular smooth muscle cells ( VSMC ) to compactin treatments.Methods VSMC were treated with different concentrations of compactin (0,1,10,20,50 μmol· L-1 ) in the absence or presence of IL -1β(20μg· L-1 ) for 24 h.Commercial kits was used to evaluated the enzymatic activity of HMGCoA reductase.Real-time PCR was used to determine the mRNA.50 μmol· L-1 com-pactin in the presence of IL -1β(20 μg· L-1 ) for 24 h was extracted, and western-blot was adopted to determine protein expression of target genes.Results Compactin inhibited enzymatic activity of HMGCoA in a dose -dependent manner and IL -1βweakened these suppressive effects.IL-1βupregulated enzymatic activity of HMGCoA reductase and was accompanied by increased HMGCoA reductase mRNA.Protein ex-pression mediated via activation of the sterol regulatory element binding protein cleavage -activating protein/sterol regulatory element binding protein -2 pathway.Conclusion Interleukin -1βcan decrease the sensitivity of vascular smooth muscle cells to compactin.
