Study of transplantation tumor suppression of metformin microbubbles enhanced epirubicin hydrochloride on human lung adenocarcinoma in nude mice
10.13699/j.cnki.1001-6821.2014.07.018
- VernacularTitle:二甲双胍微泡促进表阿霉素抑制移植瘤和及其体内分布研究
- Author:
Song-Mei LUO
1
;
Xiu-Hua ZHANG
;
Yan-Yan XU
;
Yue-Juan ZHOU
Author Information
1. 温州医科大学 附属第五医院
- Keywords:
metformin;
doxorubicin;
lung adenocarcinoma;
suppression;
distribution in the body
- From:
The Chinese Journal of Clinical Pharmacology
2014;(7):617-619
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of metformin with lipid microbubbles on human lung adenocarcinoma in nude mice.Methods A model of lung adenocarcinoma xenograft in nude mice was established.Epirubicin group and epirubicin combined with metformin microbubble group ( ECMM) were intravenously injected into drugs at the dose of 5 mg · kg -1 every 2 d.Control group and blank microbubble group were intravenously injected into blank lipid microbubbles at the dose of 0.1 mL/20 g and 0.9%NaCl every 2 d.After treatment , tumor volume was detected.Tissues were obtained at the different time ( 0.5 , 2 , 4 , 8 , 12 , and 24 h ).The inhibition rate of drug carrier system on lung adenocarcinoma as well as morphological characteristics of HE stai-ning in the nude mice were detected.Results Tumor inhibition rates of epirubicin group and ECMM group were ( 67.55 ±3.61 )% and (86.79 ±3.13 )%, respectively.Accumulation of epirubicin in heart in ECMM group was significantly lower than that in epirubicin group ( P<0.05 ).Conclusion Combination with metformin microbubbles en-hances the inhibition of epirubicin on A 549 proliferation in vitro, indica-ting relative targeting.
