Effects of etomidate on action potentials and ion channels in hippocampal CA1 neurons of rats
10.13699/j.cnki.1001-6821.2014.07.016
- VernacularTitle:依托咪酯对大鼠海马 CA1区神经元动作电位及离子通道的影响
- Author:
Chan-Juan YU
1
;
Hui JIANG
;
Chun-Yang HUANG
;
Ya-Hua LIU
Author Information
1. 新疆医科大学 附属肿瘤医院 麻醉科
- Keywords:
etomidate;
hippocampus;
action potential;
ion channel;
current clamp technique
- From:
The Chinese Journal of Clinical Pharmacology
2014;(7):611-614
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of etomidate emulsion on ac-tion potential and ligand -gated ion channel of hippocampal CA 1.Methods Forty male rats were decollated quickly , and then the hippo-campus were taken out and cut into 400 μm thickness of hippocampus slices using the slicing machine.The slices were divided into 4 groups randomly:lipid emulsion group ( n =10 ); etomidate group ( n =10 );etomidate+DIDS(4,4′-diisothiocyanato-2,2′-stilbenedisulfonic acid disodium salt hydrate ) group ( n=10 ) and etomidate+TEA ( tetraethyl-ammonium) group (n=10).The 4 groups were given lipid emulsion ,eto-midate,etomidate +DIDS ( Cl - channel blocker ) and etomidate +TEA ( K +channel blocker ) , respectively.Different stimulus intensities were give by current clamp technique and the impact of action potential firing were recorded.Results Intralipid did not change the number or ampli-tude of action potential.As the time incubated by etomidate was post-poned , the sum and amplitude of action potential in the soma of the hipp-ocampal CA1 neurons obviously reduced.When the stimulus intensity was augmented little by little , the sum of action potential firing should be notably increased but the amplitude of action potential had not obviouslychanged.DIDS plus etomidate reduced the sum of action potential after 20 min.With the stimulus intensity increasing , the degenerative time of action potential increased gradually.The last group joined with TEA which blocks potassium ion channel did not affect the suppression function of etomidate.Conclusion Etomidate emulsion may finally inhibit action potential through opening Cl -channel in order to increase Cl -internal flow to achieve general anesthesia.K+channel may have few effects on this process.
