Influence of ubenimex on immunocyte functions of mice
10.13699/j.cnki.1001-6821.2014.07.015
- VernacularTitle:乌苯美司对小鼠免疫细胞功能的影响
- Author:
Xiao-Jian DONG
1
;
Chun-Liang LIU
;
Mei GE
;
Yu-Ye XIA
;
Wen-Wei MAO
;
Xiu-Ping QIAN
Author Information
1. 上海交通大学药学院
- Keywords:
ubenimex;
tumor -bearing mouse immune function;
tumor-associated macrophages;
myeloid-derived suppressor cell
- From:
The Chinese Journal of Clinical Pharmacology
2014;(7):607-610,614
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of ubenimex on immunocyte functions of mice.Methods Antitumor activity of ubenimex was observed in implanted H22 mice.The killing activity of NK cells to target cell YAC-1, phagocytosis of peritoneal macrophage and the secretion of mac -rophage NO effect were carried out with CCK method , neutral red colori-metric method and Griess method , respectively.Lymphocyte proliferation test was done by stimulating methods in vitro and in vivo.Tumor associated macrophages(TAMs)and myeloid derived suppressor cells (MDSCs) in pe-ripheral blood and T lymphocytes , B lymphocytes and NK cell in spleen were determined by flow cytometry method.Results The 20 mg· kg-1 of ubenimex could depress the growth of H 22 and its tumor inhibitory rate was 41.71%.The killing activity of NK cells was increased by 56.5%.The 10μg· mL-1 of ubenimex significantly enhanced secretion of macrophage NO effect (P<0.01) and it also enhanced phagocytosis of peritoneal mac-rophage (P<0.05) at concentration of 100μg· mL-1.Besides the lym-phocyte proliferation was also strengthened (P<0.05).The number of T lymphocytes and CD 8+T cell in spleen was increased obviously ( P<0.01 or P<0.05).Ubenimex could adjust the number of TAMs and MDSCs in peripheral blood to normal level.Conclusion Ubenimex has antitumor growth activity through enhances non -specific immunity and specific immunity and suppresses the tumor associated cell.
