Control the quality standard of Acanthopanax
10.13699/j.cnki.1001-6821.2014.06.026
- VernacularTitle:刺五加质量标准控制
- Author:
Qi WANG
1
;
Leng-Qiu GUO
;
Bo ZHANG
;
Ya-Ping CAI
;
Ting-Li LI
Author Information
1. 黑龙江中医药大学 药学院 中药学
- Keywords:
Eleutheroside B;
Eleutheroside E;
HPLC;
macroporous resin;
standard of quality
- From:
The Chinese Journal of Clinical Pharmacology
2014;(6):553-555
- CountryChina
- Language:Chinese
-
Abstract:
Objective To purification the Acanthopanax glycosides of two kinds of main component with macroporous resin method to be control the standard of quality of Acanthopanax medicinal materials.Methods Eleutheroside B and Eleutheroside E were isolated and purified by macro-porous resin.Determination of Acanthopanax senticosus Harms of Eleuth-eroside B and Eleutheroside E content by HPLC , as a quality standard of Acanthopanax senticosus.Results The sample concentration is 0.5 g· mL-1, adsorption velocity 2 BV· h-1, 30% ethanol eluent, elution rate 1 BV· h-1.Dialysis treatment.Column:C18 (4.6 mm ×250 mm,5μm);detection wavelength was 220 nm;mobile phase:water-acetoni-trile (0~10 min, 90∶10;10~30 min,90~80∶10~20 );flow rate:1.0 mL· min -1; Column temperature was 25 ℃.Conclusion The method of operation has good reproducibility which can be used as an effective method to control the quality of Acanthopanax senticosus harms.
