Protective effects of ginkgo flavone aglycone on oxidized low-density lipoprotein induced human aortic endothelial cells apoptosis
10.13699/j.cnki.1001-6821.2014.06.024
- VernacularTitle:银杏黄酮苷元对氧化低密度脂蛋白诱导的人主动脉内皮细胞凋亡的保护作用
- Author:
Li ZHAO
1
;
Yan HE
;
Xing-De LIU
Author Information
1. 沈阳军区总医院心内科
- Keywords:
Ginkgo flavone aglycone;
atheroselerosis;
endothelial cells;
apoptosis;
P53 tumor suppressor gene;
B lymphocytes of -2 gene
- From:
The Chinese Journal of Clinical Pharmacology
2014;(6):546-549
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of ginkgo fla-vone aglycone ( GA) on human aortic endothelial cells ( HAECs) apopto-sis induced by oxidized low -density lipoprotein ( ox -LDL ) and its mechanisms .Methods The apoptosis of HAECs was detected by Ho-echst 33258 and AnnexinV -FITC/PI staining by flow cytometry using fluorescence microscope respectively.The gene expressions of P 53 tumor suppressor gene(P53) and B lymphocytes of -2 gene(Bcl-2)were as-sessed by RT -PCR.Results GA inhibited the apoptosis of HAECs markedly.The apoptotic rates were ( 22.35 ± 0.30 )%, ( 16.90 ± 0.36 )%,( 13.66 ± 0.41 )% in HAECs treated with GA at different concentrations , respectively.There was marked difference compared with the injured group ( P<0.01 ).GA can reduce transcriptional level of ap-optosis-associated genes P53 and increased Bcl-2 expression in a con-centration dependent manner after HAECs injured by ox -LDL.Conclusion GA can significantly inhibited ox -LDL -induced HAECs apoptosis , related to inhibition of NF -κB activation signaling pathway, which may be achieved by promoting the expression of Bcl-2, suppress the expression of P53.
