Effect of calcitriol combinating cisplatin on the HEC-1-A transplantation tumor of athymic mouse
10.13699/j.cnki.1001-6821.2014.06.022
- VernacularTitle:骨化三醇联合顺铂对人子宫内膜癌细胞株 HEC-1-A裸鼠移植瘤的作用
- Author:
Jian-Bo WU
1
;
Xiu ZHENG
;
Ji-Fen HU
;
Li-Hong CHEN
;
Juan-Bing WEI
;
Yi-Hong CHEN
Author Information
1. 福建医科大学附属 第一医院 妇产科
- Keywords:
adenocarcinoma endometrium;
calcitriol;
cisplatin;
trans-plantation tumor;
apoptosis rate
- From:
The Chinese Journal of Clinical Pharmacology
2014;(6):540-542
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect calcitriol combinating cisplatin on the human endometrial cancer ( HEC -1-A) transplanta-tion tumor of athymic mouse.Methods The HEC-1-A cell line was cultured in vitro.The thymic mouse with successfully tumor burden were divide into four groups:the control group (0.9%NaCl 0.25 mL), cal-citriol group (0.05 μg) , cisplatin group (50 μg) and combinating ( cal-citriol plus cisplatin ) therapy group.All of the four groups were treated once a week until 5 weeks.Results The difference of transplantation tumor volume between three therapy groups and control group is signifi-cant ( P<0.05 ).The apoptosis rate of the control , cisplati , calcitriol and combinating groups were ( 1.45 ±0.23 )%, ( 25.62 ±1.16 )%, (22.52 ±1.25 )%and (31.59 ±1.93 )%, respectively.There was sig-nificant statistical difference between combination group with the other three groups ( P<0.05 ).The mRNA expression level of Bcl -2 and Bax among the three therapy groups compared with control group were signifi-cant ( P <0.05 ).Conclusion Calcitriol had a strong anti -tumor effect in HEC-1-A transplantation tumor , the anti-tumor mechanism may associate with the Bax/Bcl-2 pathway induced HEC -1-A tumor cell apoptosis.
