Dynamic effects of Geniposide on expression of glucose transporter 4 in insulin-resistance HepG-2 cells
10.13699/j.cnki.1001-6821.2014.06.021
- VernacularTitle:栀子苷对胰岛素抵抗 HepG-2细胞葡糖转运体4表达的影响
- Author:
Chen-Ye QI
1
;
Shou-Min XI
;
Ling-Jun MA
;
Jie ZHOU
;
Shuai YUAN
;
Peng-Tao CAO
;
Shi-Peng LI
Author Information
1. 河南科技大学 医学院
- Keywords:
Geniposide;
HepG -2 cell;
insulin resistance;
glucose transporter 4
- From:
The Chinese Journal of Clinical Pharmacology
2014;(6):537-539
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Geniposide on GLUT 4 expression in insulin -resistance HepG-2 cells.Methods After estab-lish HepG-2 cell model of insulin resistance by high concentrations of insulin-induced in vitro.The cell in model were randomly divided into three groups: control , Geniposide and blank groups.The expression changes of GLUT4 in insulin resistance HepG-2 cells was observe at the time of 4, 12, 24, 36 h after culture by RT -PCR and immunocyto-chemistry.Results GLUT4 expression was first increased and then de-creased with time in HepG -2 cells after treated by Geniposide , and peaked at 8 h.However , compared with the control group , GLUT4 ex-pression was significantly improved in Geniposide group.The difference was statistically significant ( P <0.05 ).Conclusion The improving effect of Geniposide on the improvement of insulin -resistance of HepG-2 cell in vitro is related to the expression of GLUT 4.
