Determination of methotrexate concentration in human serum by HPLC
- VernacularTitle:高效效相色谱法测定人血清甲氨蝶呤的浓度
- Author:
Xin-Wen SONG
1
;
Yang WANG
;
Xin LIU
;
Qiong XU
;
Jing PENG
Author Information
1. 武汉市妇女儿童医疗保健中心 药学部
- Keywords:
methotrexate;
solid phase extraction;
HPLC
- From:
The Chinese Journal of Clinical Pharmacology
2014;(5):448-450
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid and accurate HPLC method for determination the concentration of methotrexate in human serum.Methods Chromatographic column was Hypersil C 18 column; mobile phase was methanol ,acetonitrile and phosphate buffer (2∶7∶91 ) , a flow rate was 0.8 mL· min-1;detection wavelength was 313 nm.Serum sam-ple was injected into SPE column.Methanol/acetonitrile/phosphate buff-er ( 50∶50∶250 ) was used as the eluent , and the collection of eluent was detected directly.Results The linear range of methotrexate in serum samples was 0.03-50.00 μg · mL-1 , coefficient of recoveries of low , medium and high concentrations in two groups ( group A:3.41 , 6.82 , 27.27 μg· mL-1;group B:0.05, 0.21, 0.85 μg· mL-1)were higher than 97%.Inter day and inner day RSD were less than 5%.Conclusion This method has good accuracy , high sensitivity , convenient opera-tion.It can fully satisfy the requirement of concentration monitoring of methotrexate in the pediatric clinical practice.
