Evaluation the methods for the determination of blood sirolimus concentrations and its clinical application
- VernacularTitle:西罗莫司全血浓度测定方法及临床应用
- Author:
Ming-Zhu HUANG
1
;
Xing-Jiang HU
;
Jun-Chun CHEN
;
Xing-Guang LIANG
;
Jian LIU
;
Guo-Lan WU
Author Information
1. 浙江大学 医学院 附属第一医院
- Keywords:
LC -MS/MS;
immunoassay;
sirolimus;
evaluation of the correlation;
clinical application
- From:
The Chinese Journal of Clinical Pharmacology
2014;(5):445-447
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the two analytical methods for sirolimus concentration detection in whole blood of Chinese renal transplant patients.Methods The LC -MS/MS method and chemiluminescent microparticle immunoassay (CMIA) were used to detect the whole blood sirolimus concen-trations in Chinese renal transplant patients respectively and correlation of the two methods was analyzed.Results LC-MS/MS method was charac-terized as specificity , good precision and stability , which met the require-ments of clinical testing.Statistical analysis of the two methods showed that:the correlation coefficient was 0.963 (P <0.05,n=78).An average devia-tion of 2.8 ng· mL-1 was shown when whole blood sirolimus concentrations ranging from 2 to 10 ng · mL-1 was detected using the two methods.Con-clusion There is a correlation between the LC -MS/MS and CMIA to de-termine whole blood sirolimus concentrations in Chinese renal transplant pop-ulation.An average concentration values of the same sample is higher when using CMIA determination compared with LC -MS/MS method.
