Construction of HMGN2 recombinant expression plasmid and its anti-βhepatitis virus activity
- VernacularTitle:HMGN2重组表达质粒的构建及其抗乙型病毒性肝炎的病毒活性
- Author:
Min ZHANG
1
;
Yuan ZHANG
;
Rong-Sheng TONG
Author Information
1. 四川省医学科学院
- Keywords:
high mobility group N2;
recombinant plasmid;
hepatitis B virus activity
- From:
The Chinese Journal of Clinical Pharmacology
2014;(5):432-435
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the recombinant expression vector high mobility group N 2 and explore the anti-hepatitis B virus activity of HMGN2 molecule.Methods The pET -32 a-c ( +) -HMGN2 re-combinant expression plasmid was constructed by gene clone technique.His -HMGN2 fusion protein was purified by affinity chromatography.Tricine-SDS -PAGE and Western blotting were used to identify the HMGN2 molecule.The HepG2.2.15 cell line which was infected by HBV DNA was used as a cell model.The effect of anti -HBV and HBeAg in the cell culture supernatant were determined by ELISA.And HBV DNA level in the cell culture supernatant was used to detect by real-time PCR.Results The pET-32 a-c( +) -HMG17 re-combinant expression plasmid was successfully constructed.Those fu-sion protein expression could obviously inhibited the expression of HBeAg and HBsAg;and it decreased the copy number of HBV DNA.Conclusion The HMGN2 in the cell culture supernatant has anti-HBV activity in vitro.
