Effect of Kangnaoye on angiogenesis in rats after cerebral ischemic reperfusion
- VernacularTitle:康脑液对脑缺血再灌注动物血管新生的影响
- Author:
Zhi-Min ZHAO
1
;
Zhi-Juan XIAO
;
Qian XUE
;
Yu-An ZOU
;
Li-Qiang XING
Author Information
1. 河北北方学院
- Keywords:
Kangnaoye;
cerebral ischemia reperfusion injury;
angiogen-esis;
vascular endothelial growth factor;
hepatocyte growth factor
- From:
The Chinese Journal of Clinical Pharmacology
2014;(5):429-431
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Kangnaoye on angiogene-sis in rats after focal cerebral ischemia reperfusion.Methods The male SD rats were randomly divided into 6 groups: sham operation group , model group, Kangnaoye high, middle and low dosages groups (24,12,6 g· kg -1· d-1), and nimodipine group(1 mg· kg -1· d -1).The mod-els of cerebral ischemia reperfusion were established in rats by methods of middle cerebral artery occlusion ( MCAO).Immunohistochemical method was used to observe the change of the microvessel density ( MVD ) after cerebral ischemic and the expression of vascular endothelial growth ( VEG), HGF and CD31 protein of rats which were killed at the 0.5, 3, and 7 d of reperfusion injury respectively 2 h after cerebral ischemia.The nervous function deficit scores were evaluated at the 2 , 24 , and 48 h af-ter reperfusion.Results Compared with model group , the neurological behavior performance in Nimodipine and Kangnaoye treatment groups were significantly improved , with an increased number of MVD , and en-hanced vascular endothelial growth factor ( VEGF) and hepatocyte growth factor( HGF) protein levels ( P<0.05 or P<0.01 ) , especially in the Kangnaoye middle dosage group.Conclusion Kangnaoye has neuropro-tective effect against focal cerebral ischemic injury by improving the ex-pression of VEGF and HGF , which is one of possible anti -ischemic mechanisms.
