Tetramethylpyrazine inhibits neointimia formation induced by platelet -derived growth factor through suppressing inflammatory and adhesion molecular expression
- VernacularTitle:川芎嗪下调炎症和黏附基因的表达而抑制血管新生内膜的形成
- Author:
Hong-Bing ZHANG
1
;
Qiang LI
;
Jing FENG
;
Ke-Ping DI
Author Information
1. 河北经贸大学 生物科学与工程学院
- Keywords:
tetramethylpyrazine;
vascular smooth muscle cells;
inflammation;
adhesion
- From:
The Chinese Journal of Clinical Pharmacology
2014;(4):340-343
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of tetramethylpyrazine ( TMP) on neointimal hyperplasia induced by balloon injury.Methods VSMCs were cultured by tissue explants adherent method and VSMCs proliferation models induced by platelet -derived growth factor ( PDGF) were established.Cell counting analysis was adopted to evaluate the in-hibitory effects of TMP in a dose of 0, 20, 40 and 80 μmol · L-1 on VSMCs.Western blot analysis was performed to analyze the effect of TMP on the activator protein -1 ( AP-1 ) , proliferating cell nuclear antigen ( PCNA ) , cyclooxygenase ( COX-2 ) , intercellular adhesion molecular ( ICAM -1 ) , and integrin -linked kinase ( ILK ) expressions in VSMCs.Hemetoxylin and Eosin ( HE) staining was performed to investi-gate the effects of TMP on the neointimal hyperplasia induced by balloon injury.Results TMP inhibited cell proliferation induced by PDGF ( 20μg· L-1 ) in a dose-dependent (0, 20, 40, 80 μmol· L-1 ) manner.Remarkable decreases of AP -1, PCNA, COX-2, ICAM-1 and ILK expression were demonstrated in the TMP group compared with the model group in VSMCs.TMP inhibited the neointimal thickening induced by ballon injury , with a significantly lower ratio of intima -to-media area ( l/M) in the TMP group.Conclusion TMP plays a role in preventing neointimal hyperplasia through inhibiting or blocking proliferation , expressions of inflammatory genes and adhesion molecules.
