Detection of 16 S rRNA methylase genes in clinical isolates carbapenem-resistant Klebsiella-pneumoniae
- VernacularTitle:耐碳青霉烯类抗生素肺炎克雷伯菌临床分离株中16S rRNA甲基化酶基因的检测
- Author:
Ya-Ping HANG
1
;
Chang-Xiu NING
;
Hong WANG
;
Qiao-Shi ZHONG
;
Xiao-Yan HU
;
Bai-Ling ZHANG
;
Nan ZHANG
;
Long-Hua HU
Author Information
1. 南昌大学第二附属医院 检验科
- Keywords:
16S rRNA methylase;
drug resistance;
carbapenemase;
Klebsiella-pneumoniae
- From:
The Chinese Journal of Clinical Pharmacology
2014;(4):327-331
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate existence and homology of 16 S rRNA methylase genes in carbapenem -resistant Klebsiella-pneumoniae ( CRKP ) clinical isolates.Methods Twenty -nine CRKPs were col-lected from four hospitals in Nanchang.PCR amplifications of drug resist-ant genes were performed.The horizontal transmission of the resistance plasmids was evaluated by conjugation and homology of the isolates was analyzed by pulsed field gel electrophoresis ( PFGE).Results Resistant rates of twenty -nine clinical isolates to amikacin and gentamicin were 37.9%( 11/29 ) and 69.0%( 20/29 ) , respectively.The amikacin re-sistanted isolates were simultaneously resistant to gentamicin.The exist-ence of 16 S rRNA methylase positive genes were detected in 10 isolates , including 9 strains carrying armA genes,1 strain carrying rmtB gene.All the 10 strains with 16 S rRNA methylase positive genes harbored β-lac-tamase genes and 7 strains also harbored carbapenemase genes , with blaKPC-2 and blaNDM-1 being the main genotypes.Ten experimental strains were successfully typed by PFGE and classified into 9 different genotypes and resistance plasmids were successfully transferred into the recipient E.coli J53 through conjugation experiments of 3 armA -positive isolates. Conclusion The 16S rRNA methylase gene is highly relevant to carbapenem -resistant klebsiella -pneumoniae in terms of resistance to aminoglycosides , and armA is the main genotype.Plasmids carried 16 S rRNA methylase gene can be horizontally disseminated.
