Effects of rosiglitazone on urinary protein excretion and expression of protein(synaptopodin)induced by adriamycin in glomerulosderosis rat model
10.3969/j.issn.1001-6821.2010.04.013
- VernacularTitle:罗格列酮对阿霉素致肾小球硬化症大鼠的尿蛋白排泌量及肾小球蛋白(synaptopodin)表达的影响
- Author:
Li-Qin GUO
1
;
Jing-Li TAO
;
Shang-Mei LI
;
Dong LIANG
;
De-Shen TANG
;
Xiao-Wen CHEN
;
Hua-Feng LIU
Author Information
1. 山西省长治医学院附属医院
- Keywords:
rosiglitazone;
focal segmental glomerulosclerosis;
urinary protein excretion;
glomerulosclerosis rat model
- From:
The Chinese Journal of Clinical Pharmacology
2010;26(4):292-295
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of rosiglitazone and its possible mechanism on urinary protein excretion in adriamycin-induced nephropathy rat models.Methods Rosiglitazone therapy was performed on Focal segmental glomerulosclerosis(FSGS)rat model induced by intravenous injection with adriamycin.24-hour urine protein exctetion was assessed dynamically by biuret method.The mRNA and protein expression of synaptopodin in renal cortex of each group were assayed by realtime quantitative PCR and immunohistochemistry after animals were sacririced.Results Urinary protein excretion increasing Was retarded by the treatment of rosiglitazone.Rosiglitazone treatment also partially restored the staining of synaptopodin(P＜0.05)but not affect its mRNA expression(P＞0.05).Furthermore,synaptopodin expression was positively correlated with the urinary protein excretion.Conclusion Rosiglitazone Can reduce urinary protein excretion in FSGS and its mechamism may partly relate to glomeruli synaptopodin expression up-regulaion.
