Study on the metabolites of 1, 5-dicaffeoylquinic acid in human urine by LC-ESI/MS/MS assay

VernacularTitle:液相色谱-电喷雾离子阱质谱法研究人尿中1,5-二咖啡酰奎宁酸的代谢产物
Author: Jiang-Lin LIU ¹ ; Dan YUAN ; Jian-Gang DING ; Xiao-Na DONG ; Gui-Fang DOU ; Zhuo-Na WU ; Zhi-Yun MENG
Author Information

1. 军事医学科学院野战输血研究所
Keywords: LC-ESI/MS/MS; 1,5-dicaffeoylqunie acid; metabolite
From: The Chinese Journal of Clinical Pharmacology 2010;26(3):217-220
CountryChina
Language:Chinese
Abstract: Objective To investigate metabolites of 1,5-dieaffeoylquin-ie acid (1,5-DCQA) in human urine after oral administration of 1, 5-DCQA. Methods Urine samples were collected 0-24 h after the oral administration of 600 mg dose of 1,5-DCQA, then the samples were pu-rified through C_(18) solid-phase extraction cartridge. The purified samples were analyzed by liquid chromatography and tandem electrospray ioniza-tion ion trap mass spectrometry (LC-ESI/MS/MS). Results A total of 28 metabolites were discovered, including methylated, glucuronidated and methyl-glucuronidated metabolites. Two important metabolites were determined by comparing with synthesized standards. Conclusion These results suggest that methylation, glucuronidation and isomerization are three important metabolic pathways of 1, 5-DCQA in human.