Determination of remifentanil concentration in maternal vein and umbilical artery and vein by HPLC-UV
10.3969/j.issn.1001-6821.2010.02.016
- VernacularTitle:高效液相色谱-紫外检测法测定母体静脉及新生儿脐动、静脉血内瑞芬太尼血药浓度
- Author:
Chun-Jing ZHOU
1
;
Yun YUE
;
Tie-Bing SONG
;
Sheng-Tian LUO
;
Hao WANG
Author Information
1. 天津第四中心医院
- Keywords:
HPLC-UV;
remifentanil;
blood concentration
- From:
The Chinese Journal of Clinical Pharmacology
2010;26(2):141-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC with UV method for determination of remifentanil in human blood.Methods Human blood with internal standard was extracted by butyl chloride.The sample was separated on Zobar SE-CN (4.6 mm × 250 mm,5μm) column with a mobile phase of mixture of acetonitrile,methanol and phosphate buffer,the temperature was set at 30℃ with flow rate of 1.5 mL·min~(-1).UV detection at 210 nm. Results The calibration curves were found to be linear at the range 1-20 ng·mL~(-1) of remifentanil in whole blood with a good correlation (γ=0.956).Conclusion This method is rapid,accurate and sensitive,which is very suitable for investigation of clinical blood concentration of remifentanil.
