Effect of Huoxueyizhi slice on memory impairment and Bcl-2 gene expression in hippocampus of vascular dementia rats
10.3969/j.issn.1001-6821.2010.02.013
- VernacularTitle:活血益智片对血管性痴呆大鼠记忆障碍及海马Bcl-2蛋白表达的影响
- Author:
Hui WEN
1
;
Dong JIA
;
Yue QI
;
Chang LIU
;
Yuan-Juan E
Author Information
1. 沈阳医学院,奉天医院
- Keywords:
vascular dementia;
Huoxueyizhi slice;
memory impairment;
hippocampus;
apoptosis
- From:
The Chinese Journal of Clinical Pharmacology
2010;26(2):129-132
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Huoxueyizhi slice on memory impairment and expression of Bcl-2 and Bax in hippocampus of vascular dementia(VaD)rats.Methods The imitative vascular dementia rat model was developed by repeatedly occluding the common carotid artery in combination with all abdominal injection of sodium nitroprusside(2.5 mg·kg~(-1)).The memory test,activities of SOD,MDA and Bcl-2 and Bax gene expression in hippocampus of VaD rats treated with and without Huoxueyizhi slice (1.55,3.1,6.2 g·kg~(-1)) and dihydroergotoxine (5.4 mg·kg~(-1)),continuous administration of 28 days,were measured.Results Compared with model group,Huoxueyizhi slice significantly improved memory impairment,as evaluated by shortened escape latency and increased swimming time near the platform.Meanwhile,decreased the content of MDA,increase the activity of SOD,based on the observation in hippocampal CAI region through method of the immunohistochemistry,Huoxueyizhi slice decreased the expression of the proapoptotic Bax gene,on the contrary,it increased expression of the antiapoptotic gene Bcl-2.Conclusion Huoxueyizhi slice can exert antiapoptotic effect through counter-regulating Bcl-2 and Bax gene expression.
