Experimental study of agmatine on the expression of glial fibrillary acidic protein and the levels of IFN-γ and IL-10 of allergic encephalomyelitis in rats
10.3969/j.issn.1001-6821.2010.01.012
- VernacularTitle:胍丁胺干预对变态反应性脑脊髓炎的胶质纤维酸性蛋白表达、γ-干扰素和白介素-10水平影响的实验研究
- Author:
Da-Jin PAN
1
;
Rong-Yuan ZHENG
;
Wei-Wen QIU
;
Shao-Chang WU
Author Information
1. 丽水市第二人民医院
- Keywords:
allergic encephalomyelitis;
agmatine;
astrocyte;
glial fibrillary acidic protein
- From:
The Chinese Journal of Clinical Pharmacology
2010;26(1):44-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of agmatine on the expression of glial fibrillary acidic protein(GFAP)and the levels of IFN-γ and IL-10 in experimental allergic encephalomyelitis(EAE)in rats.Meth-ods EAE was induced in Wistar rats by immunization of spinal cords homogenate of guinea pigs and complete Freund's adjuvant.Rats gived with agmatine was intrapefitoneally injected with agmtine at dosage of 25 ,50,100 mg·kg~(-1),respectively,once daily for fourteen days after im-munization.The ethologic change is observed.and the number of GFAP in central nervous system(CNS)was examined with the method of immu-nohistochemistry and the levels of IFN-γ and IL-10 of the CNS in EAE were observed with the method of sandwich-enzyme-linked inmunosorbent assay.Resuts Compared with EAE model rats,agmatine treatment sig-nificanfly caused the decrease in morbidity and the improvement clinical manifestation of rats with EAE.The number of GFAP in and around the inflamatory demylinatve lession of CNS in EAE increased obviously(P<0.05),and the levels of IL-10 had significantly increased and the levels of IFN-γ had significantly decreased,compared with model group(P<0.05 or<0.0 1).Conclusion Agmatine could promote the expression of GFAP of central nervous system in EAE.regulate the ratio of Th1/Th2 in EAE,increased the levels of IL-10 and the levels of IFN-γ in the CNS of EAE.
