Study on the reversal of multidrug resistance of human breast cancer cell line MCF-7 by siRNA in vitro
10.3969/j.issn.1001-6821.2010.01.011
- VernacularTitle:小干扰RNA逆转人乳腺癌细胞MCF-7多药耐药的体外研究
- Author:
Xiao-Ming ZHUANG
1
;
Hong XIAO
;
Zhuo WU
Author Information
1. 南京大学医学院附属鼓楼医院
- Keywords:
small interfering RNA;
breast cancer;
muhidrug resistance
- From:
The Chinese Journal of Clinical Pharmacology
2010;26(1):40-43
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study on the regulation of muihidmg resist-ance in MCF-7 cells after transfecting(small interfering RNA)siRNA targeted on MRP.Methods Small interfering RNAs(siRNA)targeted on MRP were transfected into MCF-7 cells.then the expression level of the MRP mRNA and protein was determined,the cellular sensitivity to cheomotherapeuties was detected.Results After siRNA interfering.the level of MRP mRNA and protein was decreased,meanwhile,the tumor cells'sensitivity to cheomotherapeuties obviously increased.Conclusion To some extent.the muhidmg resistance of MCF-7 cell line Was reversed.
