Determination the aqueous humor concentration of gatifloxacin solution in cataract surgery patients by HPLC-MS/MS
10.3969/j.issn.1001-6821.2009.06.011
- VernacularTitle:高效液相色谱-串联质谱法测定白内障患者应用加替沙星滴眼液后房水药物浓度
- Author:
Xuan LIU
1
;
Li MA
;
Ning-Li WANG
;
Yan-Ling WANG
;
Chen MA
;
Li-Xin GAO
;
Ying-Xiang HUANG
;
Kang WANG
;
Shi-Hong XIONG
Author Information
1. 首都医科大学附属北京同仁医院
- Keywords:
gatifloxacin;
aqueous humor drug concentration;
cataract;
HPLC - MS/MS
- From:
The Chinese Journal of Clinical Pharmacology
2009;25(6):523-525
- CountryChina
- Language:Chinese
-
Abstract:
Objective To Determine the aqueous concentration of gati-floxacin ophthalmic solution in the aqueous humor of patients undergoing cataract surgery. Methods Total 36 patients undergoing cataract surgery were divided into 4 groups randomly. All patients received topical gati-floxacin ophthalmic solution 4 doses every day for 3 days before cataract surgery. One drop of antibiotic was administered beginning 15 min prior to surgery for group 1, 30 min for group 2, 60 min for group 3, 120 min for group 4. Aqueous humor (0. 1 mL) was sampled via paracentesis. Catifloxacin concentrations were determined using validated high performance liquid chromatography/mass spectrometry procedures. Results Gatifloxacin concentrations in aqueous humor was (0. 15 ±0. 29) ,(0. 33 ±0.22), (0.31 ±0.19), (0.74 ±0.32) μg · mL~(-1) for 15, 30, 60, 120 min, respectively. Conclusion Gatifloxacin ophthalmic solution has extensive corneal penetration. The aqueous humor concentration is a-bove the minimum inhibitory concentration for important pathogens to a-chieve effective bacterial killing. Gatifloxacin concentration in aqueous humor was highest on 120 min.
