Determination of tramadol and its metabolite O-demethyltramadol concentration in human plasma and urine by RP-HPLC
10.3969/j.issn.1001-6821.2009.06.010
- VernacularTitle:反相高效液相色谱法测定曲马多及其代谢产物O-去甲基曲马多人血药及尿药浓度
- Author:
Zishun HU
1
;
Cheng WANG
;
Qin LI
Author Information
1. 天津市第一中心医院
- Keywords:
tramadol;
O - demethyltramadol;
RP - HPLC
- From:
The Chinese Journal of Clinical Pharmacology
2009;25(6):518-522
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish analytical method for the determination of tramadol and its metabolite O - demethyltramadol in human plasma and urine by RP - HPLC. Methods Separation was carried out ons a zorbax RP - select B column and the mobile phase was a mixture of 0. 05 mol · L~(-1) potassium dihydrogen phosphate ( adjusted to pH 4. 0 with phosphoric acid)-acetonitrile (90: 10). The flow rate was 1 mL · min~(-1). Fluormetric detection was performed at excitation and emission wavelengths of 216 run and 308 nm, respectively. Results In plasma, the calibration curve was linear within 12. 5 - 800. 0 ng · mL~(-1) for tramadol and 5 -320 ng · mL~(-1) for O - demethyltramadol. The absolute recoveries were all higher than 86% , and the relative recoveries were from 93% to 105%. The RSD of the within - day and between - day variations were lower than 9% and 8% , respectively. In urine, the calibration curve was linear within 12. 5 -4 000 ng · mL~(-1) for tramadol and 5 -1 280 ng · mL~(-1) for O -demethyltramadol. The absolute recoveries were all higher than 80% , and the relative recoveries were from 91% to 107%. The RSD of the within-day and between-day variations were lower than 9%. For stability test in plasma and urine, the difference of the results was within 10%. Conclusion This RP - HPLC method is simple, sensitive, accurate and specific.
