Determination of caderofloxacin concentration in healthy human plasma by RP-HPLC
10.3969/j.issn.1001-6821.2009.04.011
- VernacularTitle:反相高效液相色谱法测定卡德沙星在健康人体的血药浓度
- Author:
Nan BAI
1
;
Rui WANG
;
Yi FANG
;
Hao LIU
;
Mei-Ling YAN
;
Yong-Zeng JIN
;
Yu-Lian LI
Author Information
1. 解放军总医院
- Keywords:
RP-HPLC;
caderofloxacin;
plasma concentration
- From:
The Chinese Journal of Clinical Pharmacology
2009;25(4):334-337
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a sensitive and specific RP-HPLC method for determination caderofloxacin concentration in healthy human plasma. Methods Human plasma with internal standard was precipitated by perchloric acid. The separation was analyzed in gradient elution system and the detection wave was 286 nm. Results The linear range of pseudoephedrine was 0.1 ～ 10. 0 mg · L-1. The relative re-covery were within 96.41% ～ 109.52%. The within - day and the be-tween day RSD were all less than 15%. After single infusion cadero-floxacin of 400 mg/3 h in two healthy volunteers. It was found to be fit-ted to a two - compartment open model and its pharmacokinetic param-eters were as follows: Cmaxwas, 3.39, 4.04 mg·L-1, AUC0-t was 32.35,28.26 mg · h·L-1, t1/2β was 7.74,6.73 h, respectively.Conclusion The method is accurate, convenient, sensitive, specific,and was suitable for the study of clinical pharmacokinetics.
