Experimental study on specific glucuronidation of baicalein by liver and intestine microsomes in rats
10.3969/j.issn.1001-6821.2009.04.008
- VernacularTitle:大鼠微粒体代谢酶对黄芩素葡萄糖醛酸化代谢物影响的实验研究
- Author:
Xiao-Bin GUAN
1
;
Ling YE
;
Bi-Jun XIA
;
Zhong-Qiu LIU
Author Information
1. 广州中医药大学附属广东省中医院珠海医院
- Keywords:
baicalein;
microsomes;
metabolism
- From:
The Chinese Journal of Clinical Pharmacology
2009;25(4):322-325
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the different roles of metabolism of baicalein in rat,especial liver and intestine. Methods The enzyme re-actions by microsome incubations were employed. The content of baica-lein and baicalein- glucuronide were determined by HPLC. The glucu-ronidation rates of baicalein at different time points and at various con-centrations were measured by using 5 UGT microsomes. Results Five different microsomes are important common microsomes for glucuronida-tion of baicalein with the manner of time and concentration-dependences.Furthermore, the results also showed that jejunum microsome is the main microsome for metabolizing baicalein, and the colon microsome would be a weakest microsome for the contribution of baicalein metabolism. Con-clusion Different microsomes are responsible for the metabolism of ba-icalein in different organs/regions of the digestive system. The glucuroni-dation of baicalein in the intestine contributes significantly to that of ba-icalein in the liver.
