Efficiency and safety of haematopoietic stem cell collection in healthy donors
10.13303/j.cjbt.issn.1004-549x.2025.02.008
- VernacularTitle:健康供者造血干细胞的单采效率及安全性分析
- Author:
Rui HE
1
;
Bangqiang ZHU
1
;
Huiqin WEN
1
;
Haijing WANG
1
;
Maohong BIAN
1
;
Yujie DIAO
1
Author Information
1. Department of Blood Transfusion, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, China
- Publication Type:Journal Article
- Keywords:
peripheral blood hematopoietic stem cells;
CD34+;
apheresis efficiency
- From:
Chinese Journal of Blood Transfusion
2025;38(2):209-213
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To explore the key factors affecting the efficiency and safety of hematopoietic stem cell apheresis. [Methods] The clinical data of 59 healthy donors who underwent allogeneic hematopoietic stem cell donation in the First Affiliated Hospital of Anhui Medical University from January 2021 to June 2024 were retrospectively analyzed. The number of CD34+ cells was used to evaluate the eligibility of stem cell collection. The effects of donor gender, age, patient weight, as well as the number of WBC, MNC, RBC, Hb, HCT, PLT, CD34+ cells, CD34+ percentage and instrument operating parameters on collection efficiency were analyzed. [Results] A total of 59 donors were enrolled, and 68 occasions of stem cell apheresis were performed, with a qualified collection rate of 56%. Donor gender, age, patient weight, total blood circulation volume, anticoagulant dosage, collection time, calcium gluconate dosage and RBC, Hb, HCT levels were not significantly correlated with the collection effect (P>0.05). Multivariate logistic regression analysis showed that the number of MNC cells, CD34+ cells and stem cell product volume were the key factors affecting the efficiency and safety. A total of 12 donors had mild adverse reactions during the collection process, and all of them were improved after treatment. [Conclusion] Optimizing apheresis strategy based on the three factors of MNC, WBC count and stem cell product volume on the day of collection will help to achieve high-quality collection and improve the success rate of transplantation.
