Laboratory detection and molecular identification of a kala-azar case in Shenzhen
- Author:
HUANG Dana
;
LIU Xiaolian
;
GAO Shitong
;
LI yuan
;
TANG Yijun
;
ZHANG Qian
;
PENG Bo
;
YANG Fan
;
NIU Cong
;
ZHANG Renli
- Publication Type:Case Reports
- Keywords:
Kala-azar;
Leishmania;
laboratory detection;
genetic traceability
- From:
China Tropical Medicine
2024;24(2):218-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To perform laboratory detection and molecular traceability analysis on a case of imported kala-azar in Shenzhen to determine the infection strain. Methods Bone marrow puncture fluid and blood samples from a case of kala-azar in Shenzhen were collected for laboratory tests. The patient's bone marrow puncture fluid smears were stained with Giemsa and examined under a microscope. Blood samples were examined for antibodies using the rk39 visceral leishmania rapid diagnostic reagent. Whole blood DNA was extracted, and the ITS-1 sequence was amplified by PCR, sequenced and aligned, and a phylogenetic tree was constructed based on the ITS-1 sequence. Results Microscopic examination of the patient's bone marrow smears revealed a large number of Leishmania amastigotes without flagella, confirming the diagnosis of kala-azar. The patient's blood was tested positive with the rk39 rapid diagnostic reagent, and PCR amplification yielded an ITS-1 gene product sequence that matched the expected size. Sequence alignment with the NCBI database showed 100% sequence similarity with the ITS-1 gene sequence of Leishmania infantum, confirming the infecting strain as Leishmania infantum. Phylogenetic tree construction of the amplified ITS-1 sequence revealed clustering into a clade with Leishmania infantum , and close to KC347299, one of the reference sequence selected. Conclusions The case of kala-azar in Shenzhen was caused by Leishmania infantum. Kala-azar still occurs in China, so the diagnostic technology of medical personnel in non-epidemic areas should be strengthened so that they can actively use new diagnostic technologies to assist in diagnosis, thus improving their prevention and control ability of Leishmania parasites.
- Full text:202503071641119664618.Laboratory detection and molecular identification of a kala-azar case in Shenzhen.pdf
