Effect of chromosome-instability-associated gene GALNT7 on proliferation and apoptosis of HCT116 colon cancer cells
10.19405/j.cnki.issn1000-1492.2025.01.013
- Author:
Xiao Fang
1
;
Wei Zhao
1
;
Wenying Yu
1
;
Lingjie Pei
1
;
Wenxuan Qian
1
;
Ya Zhao
1
Author Information
1. Institute of Translational Medicine，Medical College，Yangzhou University，Yangzhou 225009；Jiangsu Key Laboratory of Experimental ＆ Translational Non-coding RNA Research，Yangzhou University，Yangzhou 225009
- Publication Type:Journal Article
- Keywords:
chromosome instability;
colorectal cancer;
GALNT7;
cell proliferation;
cell cycle;
apoptosis
- From:
Acta Universitatis Medicinalis Anhui
2025;60(1):96-101
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of chromosome instability(CIN) associated gene polypeptide N-acetylgalactosaminyltransferase 7(GALNT7) on proliferation and apoptosis of HCT116 colon cancer cells.
Methods :The HCT116 cell line withGALNT7knockdown was constructed by lentiviral infection. The correlation betweenGALNT7and CIN was verified by chromosome spread assay. The effect ofGALNT7on cell proliferation was detected by live cell counting, and the effect ofGALNT7on cell cycle distribution was detected by flow cytometry and Western blot. Caspase-3 activity and Western blot assays were used to detect the effect ofGALNT7on apoptosis.
Results :HCT116 cells showed a slower proliferation rate upon knocking down ofGALNT7, and exhibited a more scattered karyotype distribution and a phenotype of increased degree of CIN. Inhibition ofGALNT7in HCT116 cells resulted in cell cycle arrest, upregulation of P21 and downregulation of CDK6 protein levels, as well as increased levels of Caspase-3 activity, cleaved PARP1 and PUMA protein expression, and decreased levels of BCL-2 protein expression.
Conclusion :TheGALNT7gene may promote proliferation and inhibit apoptosis of HCT116 colon cancer cells through the suppression of CIN generation.
- Full text:2025030509485551251染色体不稳定相关基因GAL...CT116增殖和凋亡的影响_房晓.pdf
