Comparison of MicroScan and Phoenix Automated Systems for Detection of Extended-Spectrum beta-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae.
- Author:
Won Young JIN
1
;
Sook Jin JANG
;
Young Sook KIM
;
Geon PARK
;
Dae Soo MOON
;
Young Jin PARK
Author Information
- Publication Type:Brief Communication
- Keywords: Extended-spectrum beta-lactamase; Escherichia coli; Klebsiella pneumoniae; MicroScan; Phoenix; Double-disk synergy test
- MeSH: beta-Lactamases; Delivery of Health Care; Escherichia; Escherichia coli; Klebsiella; Klebsiella pneumoniae; Pneumonia; Polymerase Chain Reaction
- From:Laboratory Medicine Online 2012;2(1):55-58
- CountryRepublic of Korea
- Language:Korean
- Abstract: The aim of this study was to evaluate the performances of MicroScan (Siemens Healthcare, USA) and Phoenix (Becton Dickinson Diagnostic Systems, USA) automated systems for the detection of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae. ESBL-producers were detected from 18 E. coli strains and 26 K. pneumoniae strains using MicroScan, Phoenix, and double-disk synergy test (DDST). The ESBL types were determined by PCR direct sequencing. ESBL genes were detected in 38 (86.4%) of the 44 test strains. The sensitivities of MicroScan, Phoenix, and DDST were 94.6%, 79%, and 89.5%, respectively. Both MicroScan and Phoenix provided acceptable results for the examination of clinical isolates.
