Mechanism of puerarin in treatment of rheumatoid arthritis based on network pharmacology and animal experimental verification
10.19405/j.cnki.issn1000-1492.2025.01.004
- Author:
Yue Gao
1
,
2
;
Fang Tang
3
;
Wukai Ma
3
;
Weiya Lan
3
;
Zong Jiang
3
;
Zexu Jin
4
Author Information
1. Second School of Clinical Medicine，Guizhou University of Chinese Medicine，Guiyang 550002
2. Dept of Internal Medicine，Gaoyou Hospital of Traditional Chinese Medicine，Gaoyou 225699
3. Dept of Rheumatology and Immunology，the Second Affiliated Hospital of Guizhou University of Chinese Medicine，Guiyang 550003
4. Second School of Clinical Medicine，Guizhou University of Chinese Medicine，Guiyang 550002
- Publication Type:Journal Article
- Keywords:
puerarin;
rheumatoid arthritis;
CIA in rats;
network pharmacology;
Wnt / β-catenin signaling path- way;
synovial hyperplasia
- From:
Acta Universitatis Medicinalis Anhui
2025;60(1):22-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the mechanism of puerarin in the treatment of rheumatoid arthritis(RA) by network pharmacology and animal experiments.
Methods :Traditional Chinese Medicine Systems Pharmcolog Database(TCMSP) and SwissTargetPrediction database were used to collect puerarin targets, and the targets of RA were obtained from GeneCards database and OMIM database. The PPI network was established by Cytoscape 3.7.2 software. Gene ontology(GO) function and Kyotoencyclopedia of genes(KEGG) enrichment analysis were performed through the Metascape database. RA rat-collagen-induced arthritis(CIA) model was reproduced using type Ⅱ collagen emulsion, 49 Wistar rats were randomly assigned to seven groups: control group, CIA model group, low-dose, medium-dose and high-dose puerarin group, methotrexate group, Tripterysium Glycosides Tablets group. Except for the control group, the other groups were given continuous gavage for 28 days after the CIA in rats model were prepared. The redness and swelling of the joints and ankle joint pathological changes were observed in each group. Western blot was used to detect the expression of Glycogen synthase kinase3β(GSK-3β), beta-catenin(β-catenin) proteins in the synovium. Real-time quantitative polymerase chain reaction(qPCR) was used to detect the expression of GSK-3β, β-catenin and c-Myc mRNA in the synovium.
Results :Puerarin had 134 targets genes, RA had 5 821 target genes, and there were 102 overlapping target genes of puerarin and RA. It involved 184 signaling pathways, including JAK-STAT signaling pathway, NF-κB signaling pathway, Wnt signaling pathway, et al. The results of animal experiments showed that after the intervention of M-puerarin and MTX, the symptoms of redness and swelling of the hind foot were alleviated, the inflammatory cell infiltration in the synovium of the joint was significantly reduced, and the damage of cartilage and bone tissue was reduced. Compared with CIA group, the expressions of GSK-3β, β-catenin protein and GSK-3β, β-catenin and c-Myc mRNA in synovial tissue of rats after M-puerarin intervention decreased(P<0.05).
Conclusion :Puerarin has the characteristic of multi-components, multi-targets and multi-pathway intervention in RA. Puerarin may alleviate synovial hyperplasia, reduce articular cartilage erosion and bone destruction in CIA in rats by inhibiting Wnt/β-catenin signaling pathway.
- Full text:2025030416121227843基于网络药理学和动物实验探...治疗类风湿关节炎的作用机制_高月.pdf
