Extraction and identification of primary rat brain microvascular endothelial cells by improved tissue block culture method
10.19405/j.cnki.issn1000-1492.2025.01.002
- Author:
Fan Zhang
1
;
Bolin Li
2
;
Ming Chi
3
;
Haiqin Liu
4
;
Yuanyu Tang
2
Author Information
1. Dept of Acupuncture and Moxibustion，College of Acupuncture and Moxibustion，Fujian University of TCM，Fuzhou 350122
2. Basic Theory of TCM Discipline，College of TCM，Fujian University of TCM，Fuzhou 350122
3. Department of Clinical Medicine，College of Integrated Traditional Chinese and Western Medicine，Fujian University of TCM，Fuzhou 350122
4. Dept of Respiratory and Critical Care Medicine，Shanghai Changzheng Hospital Affiliated Naval Military Medical University，Shanghai 200003
- Publication Type:Journal Article
- Keywords:
brain;
microvascular endothelial cells;
primary culture;
brain microvascular tissue block culture;
fac- tor Ⅷ-related antigen;
rat;
identification
- From:
Acta Universitatis Medicinalis Anhui
2025;60(1):10-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the brain microvascular tissue block culture method for extracting primary rat brain microvascular endothelial cells and identify its effect.
Methods :Brain tissue from 4-week-old Sprague Dawley rats was screened, pre-digested and solidified to obtain brain microvascular segments. These segments were subsequently placed in a CO2incubator for primary culture. The target cells were identified by cell morphology and immunocytochemical staining for factor Ⅷ-related antigen.
Results :After a 48-hour culture periodin vitro, the short spindle cells crawled out from around the brain microvascular segments. After 72 hours, island-like cell culsters formed. After 96 hours the clusters fused and the cells formed a typical monolayer, cobble stone-like, and mosaic arrangement. Factor Ⅷ-related antigen immunocytochemical staining showed that the cytoplasm of the cells appeared brown-red, indicating positive expression; DAB stained the nucleus, showing blue-dark.
Conclusion :The brain microvascular tissue block culture method can isolate and culture primary rat brain microvascular endothelial cells.
- Full text:2025030416015829774改良组织块培养法提取原代大鼠脑微血管内皮细胞及鉴定_张帆.pdf
