Effect of hemodialysis on the biotransformation of oxo-eicosatetraenoic acids in peripheral tissues
10.12025/j.issn.1008-6358.2025.20241213
- VernacularTitle:血液透析对外周组织中氧代二十碳四烯酸生物转化的影响
- Author:
Tong LIU
1
,
2
;
Gollasch MAIK
3
;
C. Luft FRIEDRICH
4
;
Pan LIN
1
,
2
;
Jun JI
5
;
Yao MENG
5
Author Information
1. Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai 200032, China
2. Department of Nephrology, Shanghai Geriatrics Medical Center, Shanghai 201100, China.
3. Department of Internal Medicine and Geriatrics, University Medicine Greifswald, Greifswald 17475, Germany.
4. Experimental and Clinical Research Center, a joint institution of Charité Medical Faculty and Max Delbrück Center (MDC) for Molecular Medicine, Berlin 13125, Germany.
5. Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
- Publication Type:Shortarticle
- Keywords:
hemodialysis;
eicosanoids;
oxo-eicosatetraenoic acid;
biotransformation;
arteriovenous difference
- From:
Chinese Journal of Clinical Medicine
2025;32(1):93-100
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the differences of free and esterified oxo-eicosatetraenoic acids (oxo-ETEs) in blood cells and plasma from arterial and venous blood in hemodialysis (HD) patients. Methods Arterial and venous blood samples from 12 patients with end-stage renal disease (ESRD) before and after HD treatment at Charité – Universitätsmedizin Berlin, Germany, from June to December 2020 were collected. The esterified and free oxo-ETEs derived from arachidonic acid in blood cells and plasma were measured by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Results Neither esterified nor free oxo-ETEs in blood cells displayed significant arteriovenous differences before and after HD. HD predominantly affected the metabolic levels of esterified and free oxo-ETEs in plasma. HD reduced the arteriovenous differences of esterified 12-oxo-ETE, free 15-oxo-ETE, and free 5-oxo-ETE in plasma, while raised the arteriovenous differences of esterified 15-oxo-ETE. Conclusions The oxo-ETEs in blood cells are relatively well-stabilized responding to HD treatment, whereas arteriovenous differences of free and esterified oxo-ETEs in plasma are present and active in response to HD treatment, potentially contributing to the cardiovascular disease.
