Effect of Processed Polygonatum cyrtonema in Preventing Depression Induced by Chronic Unpredictable Mild Stress in Female Rats
10.13422/j.cnki.syfjx.20241216
- VernacularTitle:九制多花黄精对雌性大鼠慢性不可预知应激模型的预防效果
- Author:
Xinyu DENG
1
;
Chunhua MA
1
;
Zimeng WANG
1
;
Man TANG
1
;
Xinran LI
2
;
Lurong YU
3
;
Xianyuan HE
1
Author Information
1. College of Traditional Chinese Medicine（TCM）， Chongqing Medical University， Chongqing 401331， China
2. Chongqing Key Laboratory of Prevention and Treatment of Metabolic Diseases with TCM， Chongqing 400016， China
3. School of Chinese Materia Medica，Chongqing College of TCM，Chongqing 402760，China
- Publication Type:Journal Article
- Keywords:
depression;
prevention;
processed Polygonatum cyrtonema;
females rats;
model of depression induced by chronic unpredictable mild stress （CUMS）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(7):117-124
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the prevention and control effect of processed Polygonatum cyrtonema on depression induced by chronic unpredictable mild stress （CUMS） in female rats. MethodsForty rats were assigned into control， model， and low-， medium-， and high-dose processed P. cyrtonema groups according to the random number table method， with 8 rats in each group. The rat model of depression was established with the CUMS method. The body mass， open field test， forced swimming test， Morris water maze test， levels of neurotransmitters ［dopamine （DA）， 5-hydroxytryptamine （5-TH）， and acetylcholine （ACh）］， serum levels of sex hormones ［gonadotropin-releasing hormone（GnRH）， testosterone （T）， and estradiol （E2）］ and inflammatory factors ［tumor necrosis factor-α （TNF-α）， interleukin （IL）-6， and IL-10］， and mRNA and protein levels of factors in the brain-derived neurotrophic factor （BDNF）/tyrosine kinase receptor B （TRKB）/cAMP-response element binding protein （CREB） pathway were employed to evaluate the effect of processed P. cyrtonema on the CUMS-induced depression in female rats. ResultsThe body mass， open field test results， and forced swimming test results showed that the rat model of depression was successfully established. The comparison of behaviors， neurotransmitters， sex hormones， inflammatory factors， and neural pathways among groups showed that processed P. cyrtonema had different effects of preventing the development of depression in female rats. SPSS 25 was used for statistical analysis of error and significance. T test was conducted between groups. Each treatment group showed significant therapeutic effect compared with the model group （P<0.05）. Processed P. cyrtonema elevated the level of 5-TH （P<0.01） and lowered the levels of DA and ACh （P<0.01） in the brain tissue of female rats. In addition， it reduced the serum levels of GnRH， T， E2， TNF-α， and IL-6 （P<0.05） and up-regulated the mRNA levels of BDNF and TRKB in the rat brain. ConclusionProcessed P. cyrtonema has a non-hyperactive preventive effect on CUMS-induced depression in rats， which provides a theoretical basis for the development of processed P. cyrtonema as a functional food product.
