Study on quality evaluation of Mongolian medicine Sanzi powder：fingerprint，chemical pattern recognition and multi-component quantification analysis
- VernacularTitle:蒙药三子散质量评价研究：指纹图谱、化学模式识别和多成分分析
- Author:
Jun LI
1
,
2
;
Rongjie LI
3
;
Fengye ZHOU
3
;
Qian ZHANG
1
,
2
;
Wei ZHANG
1
,
2
;
Bohan ZHANG
4
;
Shu WANG
4
;
Xitong ZHAO
4
;
Jianping CHEN
1
Author Information
1. College of Pharmacy，Inner Mongolia Medical University，Hohhot 010110，China
2. Inner Mongolia Autonomous Region Engineering Research Center of New Pharmaceutical Screening，Hohhot 010110，China
3. Dept. of Pharmacy，the Affiliated Hospital of Inner Mongolia Medical University，Hohhot 010110，China
4. The First Clinical Medical College，Inner Mongolia Medical University，Hohhot 010110，China
- Publication Type:Journal Article
- Keywords:
Sanzi powder;
fingerprint;
chemical pattern recognition;
content determination;
quality evaluation
- From:
China Pharmacy
2025;36(4):414-420
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish fingerprint， chemical pattern recognition and multi-component quantification analysis of Sanzi powder， and evaluate its quality. METHODS HPLC method was adopted. The fingerprints of 15 batches of Sanzi powder were established by using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine （2012 edition）. Cluster analysis， principal component analysis and orthogonal partial least squares-discriminant analysis were also conducted. The variable importance in projection （VIP） value greater than 1 was used as the index to screen the differential markers， and the contents of the differential markers were determined by the same HPLC method. RESULTS A total of 21 common peaks in the HPLC fingerprints of 15 batches of Sanzi powder were calibrated， and the similarities of them were 0.994- 0.999； 6 common peaks were identified， including gallic acid （peak 3）， garminoside （peak 10）， corilagin （peak 11）， chebulinic acid （peak 16）， ellagic acid （peak 18）， crocin Ⅰ （peak 19）. According to the results of cluster analysis， YKD2024LH005，No.YKD2023LH062） principal component analysis and orthogonal partial least squares-discriminant analysis， 15 batches of samples could be clustered into two categories： S1， S5， S7， S9， S14 were clustered into one category； S2-S4， S6， S8， S10-S13， S15 were clustered into one category. VIP values of 11 differential components such as corilagin， chebulinic acid and ellagic acid were higher than 1. Among 15 batches of samples， the contents of corilagin， chebulinic acid and ellagic acid ranged 2.667-5.152， 9.506- 13.522， 0.891-1.811 mg/g. CONCLUSIONS Established HPLC fingerprint and multi-component quantification analysis of Sanzi powder are rapid and simple， and can be used for quality evaluation of Sanzi powder by combining with chemical pattern recognition. Eleven components such as corilagin， chebulinic acid and ellagic acid are differential markers affecting the quality of Sanzi powder.
