Study on Secondary Metabolites of Endophytic Fungus Fusarium sp. HSL-3 from Hainan Mangrove
10.13748/j.cnki.issn1007-7693.20222302
- VernacularTitle:海南红树林内生真菌Fusarium sp. HSL-3次级代谢产物研究
- Author:
WANG Lixia
1
,
2
;
LI Sihui
2
;
MA Yihan
2
;
WU Bin
2
Author Information
1. Changshan Agriculture Development Center, Changshan 324200, China
2. Ocean College, Zhejiang University, Hangzhou 310000, China
- Publication Type:Journal Article
- Keywords:
Mangrove;
endophytic fungus;
Fusarium sp.;
secondary metabolites;
anti-inflammatory;
anti-tumor
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(13):1759-1764
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the secondary metabolites of the endophytic fungus Fusarium sp. HSL-3 isolated from Hainan Mangrove and screen their anti-inflammatory and anti-tumor activities. METHODS This fungus was fermented statically by using rice medium, and the secondary metabolites of the fungus were isolated and purified by column chromatography and HPLC. Structures indentification of the ortained compounds was carried out through MS and NMR. RESULTS Eight compounds were respectively isolated and identified as follows:lateritin(1), 4-carbomethoxy-6-hydroxy- 2-quinolone(2), 3,5-dimethoxydihydro-fusarubin D(3), anhydrofusarbin(4), 3,3'-methylene-bis(4-hydroxybenzaldehyde)(5), crypticin B(6), vanillyl alcohol(7) and 3,4-dihydroxyphenylaceticacid(8), among which, compounds 2 and 5 were isolated from the genus Fusarium for the first time. Compounds 3 and 5 showed good anti-inflammatory activity on RAW264.7 cells with the inhibition rate of NO at 50 µmol·L-1 were 87% for compound 3 and 71% for compound 5, while compound 1 showed significant cytotoxic activity against human non-small cell lung cancer cell line A549, among which the IC50 value was (7.92±0.27) µmol·L-1. CONCLUSION Compounds 3 and 5 isolated from endophytic fungus Fusarium sp. HSL-3 isolated from Hainan Mangrove show strong anti-inflammatory activity, while compound 1 shows significant anti-tumor activity.
