Simultaneous Determination of 2 Specific Phenolic Acid in Isatidis Folium by HPLC
10.13748/j.cnki.issn1007-7693.20222333
- VernacularTitle:HPLC同时测定大青叶中2个专属性酚酸的含量
- Author:
YUAN Mingming
1
;
XIONG Xiaoli
1
;
DAI Min
1
;
ZHONG Ruijian
1
;
WU Liangfa
1
Author Information
1. Jiangxi Institute for Drug Control, NMPA Key Laboratory of Quality Evaluation of Traditional Chinese Patent Medicine, Jiangxi Province Engineering Research Center of Drug and Medical Device Quality, Nanchang 330029, China
- Publication Type:Journal Article
- Keywords:
Isatidis Folium;
HPLC;
phenolic acids;
content determination
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(14):1963-1966
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a method for the simultaneous determination of 2 specific phenolic acids glycosides 1,2,2'-tri-O-E-sinapoyl-β-gentiobiose and 1,2,6'-tri-O-E-sinapoylgentiobiose in Isatidis Folium. METHODS The HPLC analysis was carried on Elite C18 column(4.6 mm×250 mm, 5 μm) with mobile phase consisted of acetonitrile-0.1% phosphoric acid aqueous solution(gradient elution) at the flow rate of 1.0 mL·min-1. The detection wavelength was 328 nm, and the column temperature was set at 30 ℃. RESULTS 1,2,2'-tri-O-E-sinapoyl-β-gentiobiose and 1,2,6'-tri-O-E-sinapoylgentiobiose were showed good linear relationships within the ranges of 2.092-104.6 μg·mL-1(R2=0.999 6) and 2.996-149.8 μg·mL-1(R2=0.999 5). The average recovery (n=6) were 99.05% and 99.60%, RSDs were 0.34% and 0.65%, respectively. CONCLUSION The established method is simple, reliable and specific, and suitable for simultaneous determination of two components of phenolic acids glycosides in Isatidis Folium.
