Research on the mechanism by which the Y chromosome gene KDM5D inhibits the malignant progression of male bladder cancer
10.19405/j.cnki.issn1000-1492.2024.12.020
- Author:
Yuefei
;
Ran
1
,
2
,
3
,
4
,
5
;
Xudong Yao
1
,
2
,
3
,
4
,
5
Author Information
1. Shanghai Clinical College , Anhui Medical University , Shanghai 200072
2. The Fifth Clinical Medical College of Anhui Medical University , Hefei 230032
3. Dept of Urology , Shanghai Tenth People &prime
4. s Hospital , Shanghai 200072
5. The Urological Cancer Institution Afiliated to Tongji University School of Medicine , Shanghai 200072
- Publication Type:Journal Article
- Keywords:
KDM5D;
Y chromosome gene;
gender;
bladder cancer;
prognostic differences;
EMT
- From:
Acta Universitatis Medicinalis Anhui
2024;59(12):2204-2214
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of the Y chromosome gene lysine-specific demethylase 5D(KDM5D) in male bladder cancer and its effects on cancer progression, thereby explaining gender-related prognostic differences in bladder cancer.
Methods:Bioinformatics analysis was conducted to identifyKDM5Das a differentially expressed Y chromosome gene with prognostic significance in bladder cancer based on data from the TCGA database. The expression ofKDM5Din bladder cancer cell lines, collected clinical bladder cancer tissues, and adjacent normal epithelial tissues were examined using quantitative Real-time PCR(qPCR), Western blot, immunofluorescence, tumor microenvironment analysis, and bioinformatics pathway analysis. The effects ofKDM5Don bladder cancer cell proliferation and migration were validated through cell experiments followingKDM5Dknockdown and overexpression. Additionally, clinical data onKDM5Dexpression in bladder cancer tissue arrays were statistically analyzed in relation to metastasis and recurrence.
Results:KDM5Dwas generally downregulated in male bladder cancer but exhibited variable expression in different bladder cancer cell lines and between recurrent and non-recurrent bladder cancers. Overexpression ofKDM5Dinhibited tumor cell proliferation and migration, and lowerKDM5Dexpression was associated with higher recurrence and metastasis rates. Bioinformatics analysis suggested thatKDM5Dmight influence tumor metastasis or recurrence by affecting immune cell aggregation and epithelial-mesenchymal transition.
Conclusion:KDM5Dis a protective gene specific to male bladder cancer and may be associated with the generally better prognosis observed in male compared to female bladder cancer patients.
- Full text:2025022716554853664Y染色体基因KDM5D抑制...胱癌恶性进展的作用机制研究_冉越非.pdf
