Expression and distribution of μ-opioid receptors in intracardiac ganglia and effects of its agonists on atrial fibrillation
10.19405/j.cnki.issn1000-1492.2024.12.014
- Author:
Haifan Liu
1
;
Yushan Xie
1
;
Faping Wan
1
Author Information
1. Dept of Human Anatomy , Xuzhou Medical University , Xuzhou 221004
- Publication Type:Journal Article
- Keywords:
μ-opioid receptor;
intracardiac ganglia;
atrial fibrillation;
μ-opioid receptor agonists;
CX43
- From:
Acta Universitatis Medicinalis Anhui
2024;59(12):2162-2169
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression and distribution of μ-opioid receptors(MOR) in intracardiac ganglia(ICG) and the effect of its agonists on atrial fibrillation(AF).
Methods:(1) The chemical anatomical characteristics of ICG in normal SD rats were studied by immunofluorescence single staining. The expression and distribution of MOR in ICG were detected by using immunofluorescence double staining, Western blot, and RT-PCR.(2) Forty male SD rats weighing 250-300 g were randomly divided into 5 groups: normal group, AF model group(AF group), solvent control group(AF-NS group), MOR-specific agonist endomorphin-2(EM2) drug group(AF-EM2 group) and DAMGO drug group(AF-DAMGO group). The AF model was established by tail vein injection of acetylcholine(Ach) and CaCl2, and drug intervention was given during modeling. The duration of AF before and after drug administration was monitored by collecting electrocardiograms. The protein and mRNA expression of MOR in ICG and connexin 43(CX43) in atrial tissue were detected by the method of Western blot and qRT-PCR.
Results:(1) Peptide nerve fibers positive for substance P and calcitonin gene-related peptide were found in the ICG, along with parasympathetic neurons positive for acetylcholine transferase and sympathetic neurons positive for tyrosine hydroxylase. The mRNA transcripts and protein of MOR were expressed in the atrial posterior wall tissue. MOR immunoreactive products were mainly distributed in the cell bodies of ICG neurons, primarily in parasympathetic and sympathetic neurons.(2) Compared with the Normal group, the expression ofMORmRNA and protein in ICG of the AF rat decreased(P<0.05). Compared with the AF-NS group, the duration of AF was shortened in the AF-EM2 group and AF-DAMGO group, and the expression of CX43 increased(P<0.05).
Conclusion:MOR is mainly expressed in sympathetic and parasympathetic neurons in ICG. The expression of MOR decreases in AF rats. MOR agonists alleviate AF.
- Full text:2025022716132411320μ-阿片受体在心内神经节的...分布及其激动剂对房颤的作用_刘海帆.pdf
