Study on Mechanism of Fermentation of Astragalus Membranaceus on Serum Metabonomics in Hyperuricemia Based on UHPLC-HRMS
10.13748/j.cnki.issn1007-7693.20221106
- VernacularTitle:基于UHPLC-HRMS血清代谢组学研究黄芪发酵菌质干预高尿酸血症的作用机制
- Author:
GE Xueli
1
;
WANG Yuqi
2
;
ZHANG Wenwen
1
;
SHI Zhongqi
1
;
TAO Yufan
1
;
LIN Zhaozhou
3
;
SU Zhenguo
4
;
ZHANG Jiayu
1
Author Information
1. Binzhou Medical University, Yantai 264003, China
2. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
3. Beijing Zhongyan Tongrentang Pharmaceutical Research and Development Co., Ltd., Beijing 100075, China
4. Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264100, China
- Publication Type:Journal Article
- Keywords:
fermentation of Astragalus membranaceus;
hyperuricemia;
serum metabolomics;
mechanism
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(14):1897-1905
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To research the effect and mechanism of fermentation of Astragalus membranaceus on endogenous metabolites in hyperuricemia model rats using serum UHPLC-HRMS. METHODS The SD rats were randomly divided into different groups, including blank group, model group, benzbromarone group(20 mg·kg-1), as well as fermentation of Astragalus membranaceus high-dose(3 g·kg-1) and low-dose group(1.5 g·kg-1). Model group and each treatment group were disposed with 300 mg·kg-1 oxonic acid potassium to establish hyperuricemia models. At the time of 1 h after modeling, rats in each treatment group were given corresponding drugs for intervention. Collected rat serum after 14 d. The serum of different groups were collected for endogenous metabolites research using UHPLC-HRMS. After multivariate statistical analysis, the different metabolites and metabolic pathways were selected. RESULTS The hyperuricemia rat modes were successfully established by oxonic acid potassium 14 d, and fermentation of Astragalus membranaceus showed good uric acid reducing effect. Compared with the blank group, 17 potential biomarkers associated with hyperuricemia were found in the model group. Among them, 9 potential biomarkers were significantly recalled by fermentation of Astragalus membranaceus. It mainly involved sphingolipid metabolism, pyrimidine metabolism, tryptophan metabolism, pantothenic acid and CoA biosynthesis, glycine, serine and threonine metabolism and other pathway. CONCLUSION This study can provide a basis for revealing the mechanism of reducing uric acid by fermentation of Astragalus membranaceus, and lay a foundation for the further development and utilization of Astragalus.
