Study on Application of Microfluidic Chip Rapid Detection in the Detection of Pesticide Residues in Dendrobium Officinale
- VernacularTitle:微流控芯片快速检测技术在铁皮石斛农药残留检测中的应用研究
- Author:
QIU Yijing
1
;
JIA Yanbo
1
;
ZHANG Jiafang
1
;
CHEN Lifang
1
Author Information
- Publication Type:Journal Article
- Keywords: microfluidic chip; Dendrobium officinale; rapid detection; pesticide residues
- From: Chinese Journal of Modern Applied Pharmacy 2023;40(15):2131-2139
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE To explore the feasibility of microfluidic chip in the rapid detection of pesticide residues in Dendrobium officinale. METHODS The sample was extracted with organic solvent, and purified with QuEChERS dispersive SPE, high throughput screening of enzymes for pesticide residue detection from different manufacturers by centrifugal microfluidic chips. The matrix interference was investigated by negative samples with different horizontal adding methods. The sensitivity, repeatability sand accuracy of the microfluidic chip were investigated, and sample results determined by mass spectrometry were compared with them. RESULTS The results revealed that the significant difference rate of rapid detection method was 0.25; the sensitivity was 99.6%; the specificity was 96.4%; the false negative rate was 0.39%; the false positive rate was 3.57%; the accuracy was 98.81%. Two methods of rapid detection of pesticide residues by microfluidic chip and mass spectrometry were used to detect 40 batches of samples. The results indicated that the two methods had a high consistency, compliance rate of testing results were 100%. CONCLUSION Microfluidic chip rapid detection technology can be used for detecting pesticide residues in Dendrobium officinale, which can meet the screening needs of the basic non-professional person to detect the quality of large quantities of pesticide residues.
