Study on Inflammatory Immune Mechanism of Lactoferrin in the Treatment of Periodontitis
10.13748/j.cnki.issn1007-7693.20223405
- VernacularTitle:乳铁蛋白治疗牙周炎的炎症免疫机制研究
- Author:
XU Junfeng
1
;
XU Wanjun
1
;
DONG Yanrong
1
;
DENG Zuyue
2
;
JIANG Xia
2
;
YUAN Ying
1
;
FANG Jianhong
1
;
WAN Yue
3
;
REN Yanyun
3
Author Information
1. Tongde Hospital of Zhejiang Province, Hangzhou 310012, China
2. Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China
3. No.903 Hospital of PLA, Hangzhou 310013, China
- Publication Type:Journal Article
- Keywords:
lactoferrin;
periodontitis;
NLRP3 inflammasome;
anti-inflammatory;
inflammatory factor
- From:
Chinese Journal of Modern Applied Pharmacy
2023;40(15):2086-2092
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the anti-inflammatory immune response effects of lactoferrin in the treatment of periodontitis and its mechanism. METHODS One hundred SD rats were randomly divided into blank control group, model group, lactoferrin administration group low, medium, high dose group(1, 2, 3 g·kg-1), metronidazole positive control group (0.02 g·kg-1), PDTC group(200 mg·kg-1), lactoferrin+PDTC group(2 g·kg-1, 200 mg·kg-1), MCC950 group(1 mg·kg-1) and lactoferrin+MCC950 group(2 g·kg-1, 1 mg·kg-1), 10 rats in each group. Silk thread ligation combined with 10% sucrose drinking water was used to establish the model, and then the drug was administered orally once a day. The blank control group and the model group were administered orally with 0.9% NaCl. The rats in each group were sacrificed after one month of continuous administration. The contents of IL-1b, IL-8 and IL-10 were detected by ELISA kit, and the expressions of TLR2-NF-κB pathway and NLRP3 inflammasome related proteins were detected by Western blotting. HE staining was used to observe the pathological changes of the periodontal tissues of the rats in each group. RESULTS Compared with the model group, the symptoms of periodontitis in each dose group of lactoferrin were significantly improved. HE staining showed that the infiltration of inflammatory cells was reduced, and the proliferation of fibroblasts was active. The protein expressions of TLR2, NF-κB, NLRP3, Caspase-1 p20 and GSDMD-N decreased, the content of pro-inflammatory factor IL-8 and IL-1b decreased, and the content of anti-inflammatory factor IL-10 increased. CONCLUSION Lactoferrin may play a role in the regulation of inflammatory immune response in the treatment of periodontitis by down-regulating the protein expression of TLR2-NF-κB-NLRP3 pathway, reducing the initiation of inflammatory response and the release of inflammatory factors, so as to achieve the purpose of anti-inflammatory.
