Fabrication and research of chitosan-β-TCP composite lamellas scaffold
10.19405/j.cnki.issn1000-1492.2022.07.020
- Author:
Yifan Lu
1
,
2
,
3
;
Duohong Zou
4
,
5
,
6
,
7
;
Ruyuan Ding
1
,
2
,
3
;
Aibing Hou
1
,
2
,
3
Author Information
1. Stomatological College of Anhui Medical University，Hefei 230032
2. The Affiliated Stomatological Hospital of Anhui Medical University，Hefei 230032
3. Key Lab of Oral Diseases Research of Anhui Province，Hefei 230032
4. Stomatological College of Shanghai Jiao Tong University，Shanghai 200001
5. Dept of Oral Surgery of Shanghai Jiao Tong University Affiliated Shanghai Ninth People&rsquo
6. s Hospital，Shanghai 200001
7. Shanghai Key Laboratory of Stomatology ＆ Shanghai Research Institute of Stomatology，Shanghai 200001
- Publication Type:Journal Article
- Keywords:
Chitosan;
β-TCP;
composite scaffold;
bidirectional lyophilization technique;
osteogenic differentia- tion
- From:
Acta Universitatis Medicinalis Anhui
2022;57(7):1122-1127
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanical property,biocompatibility,and osteogenic differentiation ability of chitosan-β-TCP( CS-β-TCP) scaffold,meanwhile,to study the possibility of the composite as a scaffold to repair bone defect.
Methods:Briefly,the CS-β-TCP composite scaffold was fabricated utilizing bidirectional lyophilization technique. Then,the scaffold micro-structure was observed by scanning electron microscopy( SEM),X-ray diffraction( XRD) and energy disperse spectroscopy( EDS) were employed to analyze the ingredients and elements distribution of scaffold,respectively. Additionally,the compression strength of the scaffold was tested by mechanical universal testing machine. The biocompatibility of the scaffold and the cell viability research were characterized via CCK-8 assay and Live/Dead staining,respectively,and the cell adhesion was studied by DPAI/Phalloidine fluorescence staining. qRT-PCR was employed to investigate the expression level of osteogenic-related gene such as BMP2,RUNX2 and COL1. ALP staining was carried out to measure the osteogenic differentiation effect of BMSCs.
Results:The CS-β-TCP scaffold was comprised of bulk parallel,aligned and thin lamellas with many porous structures. β-TCP particles were evenly distributed over CS framework layers and the CS-β-TCP scaffold possess excellent elastic property and biocompatibility,moreover,the cell seeded on scaffold revealed high cell viability and continuous proliferation. qRT-PCR and ALP staining results demonstrated that the CS-β-TCP scaffold could induce osteogenic differentiation of BMSC.
Conclusion:To sum up,the CS-β-TCP scaffold expressed desired mechanical and biological properties,and could induce BMSC differentiate into osteoblast,the composite scaffold provided a promising strategy for bone defect regeneration.
- Full text:2025022709291033770壳聚糖-β-磷酸三钙复合层状支架的制备与研究_陆依凡.pdf
