Mechanism of liposome curcumol reversing cisplatin resistance in ovarian cancer
10.19405/j.cnki.issn1000-1492.2022.07.017
- Author:
Jiezhen Yang
1
;
Jing Wang
2
;
Yonghong Song
3
;
Qin Tang
1
;
Qiang Wu
4
,
5
Author Information
1. Dept of Pathology，Anhui Medical University，Hefei 230032
2. Dept of Obstetrics and Gynecology，The First Affiliated Hospital of Anhui Medical University，Hefei 230022
3. School of Chemistry and Chemical Engineering，Hefei University of Technology，Hefei 230009
4. Dept of Pathology，Anhui Medical University，Hefei 230032
5. Dept of Pathology，The First Affiliated Hospital of Anhui Medical University，Hefei 230022
- Publication Type:Journal Article
- Keywords:
ovarian cancer;
cisplatin resistance;
liposome curcumol;
P-gp
- From:
Acta Universitatis Medicinalis Anhui
2022;57(7):1106-1111
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and mechanism of liposome curcumol( LC) on cisplatin resistance of human ovarian cancer cells.
Methods:Ovarian cancer cisplatin resistant cell line SKOV3/DDP was used. Cells were divided into five groups: blank control group,negative control group( containing 12. 5 μg/ml liposome),cisplatin group( 4 μg/ml),LC group( 20 μg/ml),and combined group( cisplatin( 4 μg/ml) + LC( 20 μg/ml).Cell proliferation was detected by CCK8 assay,the content of cisplatin in cells was detected by high performance liquid chromatography-tandem mass spectrometry( HPLC-MS),and apoptosis was detected by flow cytometry. The level of P-glycoprotein( P-gp) mRNA was detected by qRT-PCR and the expression of P-gp protein was detected by immunohistochemistry and Western blot.
Results:The cell proliferation ability of the combination group was the lowest while the apoptosis rate was the highest( P<0. 05) among the groups. HPLC-MS showed that the content of cisplatin in combined group was higher than that in cisplatin group( P<0. 05). P-gp mRNA and protein in the combined group were down-regulated( P<0. 05).
Conclusion:LC can reduce cisplatin resistance of human ovarian cancer cell line SKOV3/DDP by inhibiting the expression of P-gp.
- Full text:2025022709134881049脂质体莪术醇逆转卵巢癌顺铂耐药作用的机制_杨洁真.pdf
