Over-expression of circRNA ubiquitin ligase E3 (circHERC4/hsa＿circ＿0007113) inhibits cell senescence
10.19405/j.cnki.issn1000-1492.2022.07.016
- Author:
Hualing Li
1
;
Di Yang
1
;
Junjie Wei
1
;
Zhangyue Chen
1
;
Juping Chen
2
Author Information
1. Dept of Biochemistry and molecular biology，Medical College，Yangzhou University，Yangzhou 225001
2. Dept of Dermatology，Affiliated Hospital of Yangzhou University，Yangzhou 225001
- Publication Type:Journal Article
- Keywords:
circRNA;
senescence;
ubiquitin ligase 4 circRNA (circHERC4 hsa_circ_0007113);
human embry- onic lung fibroblasts cells (IMR-90)
- From:
Acta Universitatis Medicinalis Anhui
2022;57(7):1100-1105
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship between the ubiquitin ligase E3 cirRNA(circHERC4/hsa＿circ＿0007113) and the senescence in Human embryonic lung fibroblasts(IMR-90)cells.
Methods:The whole gene sequence of circHERC4 was obtained from circBase database and cloned into cirRNA expression vector pLC5-ciR. The recombinant pLC5-ciR(+) hsa＿circ＿0007113 was constructed in vitro. The recombinant plasmid was identified by PCR, enzyme digestion and sequencing. The recombinant plasmid was transfected into HEK293 T and IMR90 cells with liposome 2000 transfection reagent. The expression of circHERC4 in normal cells, empty vector group and recombinant vector group was detected by RT-PCR, and the senescence of cells was detected by SA-β-Gal staining. Cell proliferation was detected by CCK-8 method.
Results:The overexpression vector of circHERC4 was successfully constructed by correct sequencing, and circHERC4 could be efficiently transfected in HEK293 T cells. Compared with the control group, the positive rate of SA-β-Gal staining in the recombinant vector group decreased(P<0.05), and the proliferation rate of cells increased(P<0.05). Overexpression of circHERC4 could improve the proliferation and inhibit the senescence in IMR90 cells.
Conclusion:It suggests that circHERC4 has potential function of anti-senescence, which lays a foundation for further study on the function of circHERC4.
- Full text:2025022709052848398过表达环状RNA泛素连接酶...113)抑制细胞衰老的研究_李华玲.pdf
