Detection of structural characteristics of hypothalamic-pituitary-adrenal axis in macaques
10.19405/j.cnki.issn1000-1492.2022.07.015
- Author:
Tingyu Dong
1
;
Menghui Guo
1
;
Changyong Xu
1
;
Haifeng Jiang
1
;
Lei Zhang
1
;
Zhen Xu
1
;
Xiaoyi Liu
1
;
Shangxue Yan
1
;
Yan Chang
1
;
Wei Wei
1
Author Information
1. Institute of Clinical Pharmacology，Anhui Medical University，Key Lab of Anti-inflammatory and Immune Medicine，Ministry of Education，Rheumatoid Arthritis Research Center，Anhui Medical University，Hefei 230032
- Publication Type:Journal Article
- Keywords:
macaques;
hypothalamic-pituitary-adrenal axis;
pathological section;
HE staining;
immunohistochemistry;
immunofluorescence
- From:
Acta Universitatis Medicinalis Anhui
2022;57(7):1094-1099
- CountryChina
- Language:Chinese
-
Abstract:
Abstract:To observe the histomorphological features of the hypothalamic-pituitary-adrenal axis in macaques to provide a reference for simulating the physiological functions and pathological responses of the human hypothalamic-pituitary-adrenal axis.
Methods:After euthanasia of macaques, hypothalamus, pituitary and adrenal tissues were removed intact, fixed by PFA, and paraffin sections and frozen sections were prepared; the basic structure and cellular distribution were observed by HE staining; the secreted hormones and receptors were detected by immunohistochemistry; the effects of staining in frozen and paraffin sections were compared, and the cellular composition of some hypothalamus tissues was identified.
Results:The hypothalamic region was hollow and funnel-shaped, the pituitary gland resembles a pea, and the right and left adrenal glands were located between the liver and kidneys; HE staining showed that the hypothalamic region was mainly composed of neurons and microglia, the pituitary gland was divided into neuro-pituitary and adeno-pituitary, and the adrenal gland was composed of cortex and medulla; immunohistochemical results showed that the hypothalamus secretes CRH and expresses GR, the pituitary gland secretes ACTH and expresses CRHR1 and GR, and the adrenal gland expresses ACTHR; immunofluorescence of frozen sections better showed that the hypothalamus contains neurons and microglia.
Conclusion:In this study, sections of hypothalamus, pituitary and adrenal gland tissues from macaques were successfully produced, and the relevant anatomical and morphological features were observed and examined, which provided a reference method for simulating the physiological and pathological responses of the human hypothalamic-pituitary-adrenal axis.
- Full text:2025022709004544945猕猴下丘脑-垂体-肾上腺轴组织结构学特征观察_董婷玉.pdf
