Histologic Characteristics in the Eyes of DBA/2J Mice according to the Degree of Intraocular Pressure.
- Author:
Myoung Hee PARK
1
;
Do Hyun KIM
;
Yong Woo IM
;
Jung Il MOON
Author Information
1. Department of Ophthalmology, College of Medicine, The Catholic University of Korea, Seoul, Korea. jimoon@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
DBA/2J mice;
Glaucoma;
Intraocular pressure;
Mouse model;
Ocular abnormality
- MeSH:
Anesthesia;
Animals;
Atrophy;
Eosine Yellowish-(YS);
Ganglion Cysts;
Glaucoma;
Hematoxylin;
Intraocular Pressure*;
Iris;
Mice*;
Mice, Transgenic;
Retina;
Retinal Ganglion Cells;
Transillumination
- From:Journal of the Korean Ophthalmological Society
2004;45(9):1547-1553
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We assessed the correlation between ocular abnormalities and the degree of intraocular pressure (IOP) in the DBA/2J (D2) transgenic mice which were proven to have pigmentary dispersion syndrome and developing glaucoma. METHODS: Nine-months-old D2 mice were examined with biomicroscopy under anesthesia and measured for IOP by Tono-Pen, Hematoxylin and eosin staining was performed on the eye sections of the mice to analyze differences between the low-grade IOP group and the high-grade IOP group. RESULTS: Ocular abnormalities including iris pigment loss, iris transillumination, iris stromal atrophy, anterior synechia, thinning of the retina, and ganglion cell loss were found; all of which appeared to be pressure- dependent. CONCLUSIONS: These results corroborate that both IOP and age might be considered for studies using D2 mice, and suggest that D2 mice are a useful glaucoma model to study the mechanisms of retinal ganglion cell death and to evaluate strategies for neuroprotection.
