Expression and effect of miR-30b in the trigeminal neuralgia model rats
10.19405/j.cnki.issn1000-1492.2022.07.012
- Author:
Tingting Hu
1
,
2
,
3
;
Fan He
1
,
2
,
3
;
Mingzheng Liu
1
,
2
,
3
;
Wenhua Xu
1
,
2
,
3
;
Yuanying Wang
1
,
2
,
3
Author Information
1. Stomatologic College，Anhui Medical University，Hefei 230032
2. The Affiliated Stomatological Hospital of Anhui Medical University，Hefei 230032
3. Key Lab．of Oral Diseases Research of Anhui Province，Hefei 230032
- Publication Type:Journal Article
- Keywords:
Trigeminal neuralgia;
infraorbital nerve-chronic constriction injury;
miR-30b;
ATF3
- From:
Acta Universitatis Medicinalis Anhui
2022;57(7):1078-1083
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of miR-30b in the trigeminal ganglion( TG) of trigeminal neuralgia( TN) model rats.
Methods:An infraorbital nerve-chronic constriction injury( ION-CCI) model of TN was produced in the rat. Von Frey hair was used to determine the time-course of changes in mechanical pain threshold.qRT-PCR and Western blot were used to detect the expression of miR-30b and activating transcription factor 3( ATF3) in the TG. In addition,miR-30b agomir and agomir NC were injected into ION-CCI rats by the infraorbital foramen injection. The mechanical pain threshold and the expression changes of ATF3 were determined after intervention.
Results:Compared with the sham-operated group,the ION-CCI group had a decreased mechanical pain threshold 2 days after surgery,reached the lowest observed level by day 12,and this threshold reduction lasted more than 28 days( P<0. 05). Compared with the sham-operated group,the ION-CCI group had a lower miR-30b expression and a higher ATF3 expression in postoperative TG( P<0. 05). After the overexpression of miR-30b in ION-CCI group,the mechanical pain threshold increased,and the expression of ATF3 decreased in TG( P<0. 05).
Conclusion:These results suggest that miR-30b may participate in the regulation of TN. miR-30b may be a potential therapeutic target for TN.
- Full text:2025022708460377296miR-30b在三叉神经痛模型大鼠中的表达及作用_胡婷婷.pdf
