Expression of UGRP1 in thyroid cells induced by IL-1β and its correlation with Fas/FasL mediated apoptosis
10.19405/j.cnki.issn1000-1492.2022.07.011
- Author:
Cuiping Chen
1
;
Anan Li
1
;
Cuiping Ren
2
;
Jijia Shen
2
;
Chunlin Zuo
1
Author Information
1. Dept of Endocrinology，The First Affiliated Hospital of Anhui Medical University，Hefei 230022
2. Dept of Microbiology and Parasitology，School of Basic Medical Sciences，Anhui Medical University，Hefei 230032
- Publication Type:Journal Article
- Keywords:
interleukin-1 β;
uteroglobin-related protein 1;
Fas / FasL pathway;
apoptosis;
autoimmune thyroid dis- eases
- From:
Acta Universitatis Medicinalis Anhui
2022;57(7):1073-1077
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of uteroglobin-related protein 1(UGRP1) in thyroid cells induced by interleukin-1β(IL-1β) and its correlation with Fas/FasL mediated apoptosis.
Methods:Control group, IL-1β group and IL-1β+anti-FasL antibody group were established, rat thyroid cells(FRTL-5 cells) were culturedin vitro. The expression levels of UGRP1 and Fas mRNA of each group were detected by real-time PCR and the apoptosis rate of each group was detected by flow cytometry.
Results:Compared with control group, the mRNA expression levels of UGRP1 and Fas in IL-1β group and IL-1β+anti-FasL antibody group increased, and the difference was statistically significant(P<0.05). Compared with control group, the early apoptosis rate of thyroid cells in IL-1β group increased(7.49%±1.91%vs28.46%±3.17%), and the difference was statistically significant(P<0.001). Compared with IL-1β group, the early apoptosis rate of thyroid cells in IL-1β+anti-FasL antibody group decreased(28.46%±3.17%vs19.20%±1.75%), and the difference was statistically significant(P<0.05). Compared with IL-1β group, the expression levels of UGRP1 mRNA(2.22±0.31vs2.66±0.28) and Fas mRNA(2.75±0.18vs3.03±0.16) in IL-1β+anti-FasL antibody group were not significantly different(P>0.05).
Conclusion:IL-1β induces the high expression of UGRP1 and Fas and apoptosis of thyroid cells, the high expression of UGRP1 is not associated with Fas/FasL mediated apoptosis.
- Full text:2025022708405929662IL-1β诱导甲状腺细胞U..._FasL介导凋亡的相关性_陈翠萍.pdf
