Study of sodium distribution in rats with nephrotic syndrome
10.19405/j.cnki.issn1000-1492.2022.07
- Author:
Lianghou Ni
1
,
2
;
Mo Yang
1
,
2
;
Manman Li
1
,
2
;
Yunlai Wang
1
,
2
;
Zihua Xuan
1
,
2
;
Fan Xu
1
,
2
Author Information
1. College of Pharmacy，Anhui University of Chinese Medicine，Hefei 230012
2. Key Laboratory of Chinese Medicine Formula of Anhui Province，Hefei 230012
- Publication Type:Journal Article
- Keywords:
nephrotic syndrome;
sodium retention;
lymphatic;
skin;
sodium
- From:
Acta Universitatis Medicinalis Anhui
2022;57(7):1054-1059
- CountryChina
- Language:Chinese
-
Abstract:
Abstract:To investigate the locations of sodium retention and the mechanism of its occurrence in the rat model of adriamycin nephrotic syndrome.
Methods:The nephrotic syndrome model was established by tail vein injections of adriamycin twice two weeks(4 mg/kg in first week and 2 mg/kg in second week). Urine and blood biochemical parameters were detected; the contents of water and sodium in rat skin were detected respectively by desiccation and atomic absorption spectrometry; the content of glycosaminoglycan was analyzed by ELISA; the expression of tonicity-responsive enhancer binding protein(TonEBP), vascular endothelial growth factor C(VEGFC), vascular endothelial growth factor receptor 3(VEGFR3) and lymphatic vessel hyaluronan receptor-1(LYVE-1) in the renal cortical was detected by Western blot; the lymphatic vessels distribution in the renal cortex was measured by immunofluorescence.
Results:the model rats had increased urinary protein excretion, and abnormal renal function and lipid, which suggested the model was successfully established. The excretion of Na+in urine decreased, the content of skin sodium and glycosaminoglycan in skin obviously increased, the expression of TonEBP, VEGFC, VEGFR3, LYVE-1 in renal cortical markedly increased, and density of lymphatic vessels in renal cortical notably increased(P<0.05 orP<0.01).
Conclusion:Sodium retained in nephrotic syndrome rats may be stored in the skin, which is related to the TonEBP/VEGFC/VEGFR3 pathway.
- Full text:2025022617044933148肾病综合征大鼠体内钠离子分布研究_倪良厚.pdf
